RAPD primer screening for markers development of Jinten Hitam (Nigella sativa L.) authentication

Jinten hitam ( Nigella sativa ) is one of the medicinal plants in family Ranunculaceae, native to Mediterranean region and several regions Asia. The seed widely cultivated, distributed, used pharmaceuticals, cosmetics, food industries. Due similar morphology its potential adulterant such as Corchorus spp., N. seeds are susceptible adulteration substitution markets. Molecular markers have become most reliable methods for identification authentication plants. objective this study was select random amplified polymorphic DNA (RAPD) primer generating ). Genomic extracted from samples seed, sp. a mixture both samples. Forty-two RAPD primers were study. A total 227 fragments produced 37 primers, out which 65% polymorphic. Primer OPK-4 OPC-12 generated specific , meanwhile, OPB-1, OPL-5, OPM-3, OPD-5, . molecular marker able using selected primer. This research first report on screening spp.).